Intragenotypic variability of Capsicum chinense Jacq. “ají Supano” from the lower river basin of the Supe- Barranca
DOI:
https://doi.org/10.32911/as.2018.v11.n2.579Keywords:
ISSR, Capsicum chinense Jacq, chili Supano, BarrancaAbstract
Capsicum chinense “ají supano” is an ecotype typical of the countryside of Supe very
appreciated in the regional cuisine for its aroma and flavor. This ecotype to date has
no studies, so the objective of the present study is to characterize it molecularly to
identify intragenotypic variability of 30 individuals of chili peppers from the lower
Supe river basin using molecular markers ISSR. Fruits were collected from four
farmers during the month of November, 2016, being the seeds selected randomly,
for their establishment in pots in house mesh. DNA was extracted from three young
leaves using the Micro - CTAB method modified by Biotechnology Lab of PIPS in
Cereals and Native Crops of UNALM to molecular analysis. Primers SSR 22, UCB
807, UCB 810 and UCB 841 were selected due their high resolution and banding;
which did not detect different bands among the individuals evaluated, confirming that
it is an autogamous ecotype, all the individuals of the population are genetically equal,
therefore there is no intragenotypic variability
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References
Aguilera, Jorge et al. 2011. Genetic variability by ISSR markers in tomato (Solanum lycopersicon Mill). Rev. Bras. Ciênc. Agrár. 6: 243-252.
Andreev, Oscar et al. 2005. Variability of ribosomal RNA genes in Rauwolfia species: Parallelism between tissue culture-induced rearrangements and interspecies polymorphism. Cell Biol. Intl.. 29:21–27.
Arif, Ibrahimi et al. 2010. A brief review of molecular techniques to assess plant diversity. Int. J. Mol. Sci. 11: 2079 - 2096.
Astarini, Ida et al. 2006. Genetic diversity of Indonesian cauliflower cultivars and their relationships with hybrid cultivars grown in Australia. Scientia Horticulturae. 108: 143- 150.
Bosland, Paul y Votava Eric. 2012. Peppers: vegetable and spice capsicums. 2nd (Ed.). Cabi publishing. London UK. 230 p.
Christensen, T. et al. 2007. Drosophila MCM10 in heterochromatin dynamics and DNA replication. A. Dros. Res. Conf. 48 : 139.
Del Rio, A. et al. 2001. Association of ecogeographical variables and RAPD marker variation in wild potato populations of the USA. Crop Sci. 41: 870-878.
Deshpande, AU. y otros 2001. Genetic diversity across natural populations of montane plant species from the Western Ghats, India reveled by intersimple sequence repeats. Molecular Ecology. 10: 2397–2408.
Dogan, B.; Duran, A. y Hakki. E. 2007. Phylogenetic analysis of Jurinea (Asteraceae) species from Turkey based on ISSR amplification. Annal. Bot. Fennici 44: 353-358.
Doyle, J. y Doyle, L. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull. 19:11-15.
Eshbaugh, W. 1970. A biosystematic and evolutionary study of Capsicum baccatum (Solanaceae). Brittonia. 22: 31–43.
Fekadu, M. et al. 2008. Genetic components and heritability of yield and yield related traits in hot pepper. Research Journal of Agriculture and Biological Science. 4: 803 - 809.
Font, C. et al. 2014. Phenotypic diversity among local Spanish and foreign peach and nectarine (Prunus persica L.) Batsch accessions. Euphytica. 197(2): 261-277.
Galvan, M.; Bornet, B.; Balatti, P. y Branchard, M. 2003. Inter simple sequence repeat (ISSR) marker as a tool for the assessment of both genetic diversity and gene pool origin in common bean (Phaseolus vulgaris L.). Euphytica.132: 297-301.
Gernand, D. et al. 2007. Tissue culture triggers chromosome alterations, amplification, and transposition of repeat sequences in Allium fistulosum. Genome. 50: 435–442.
Girma, G.; Tesfaye K. y Bekele E. 2010. Inter Simple Sequence Repeat (ISSR) analysis of wild and cultivated rice species from Ethiopia. African Journal of Biotechnology. 9: 5048-5059.
Heiser, C. y Pickersgill, B. 1969. Names for the cultivated Capsicum species (Solanaceae). 18: 277–283.
Ince, A.; Karaca M. y Onus, A. 2010. Genetic relationships within and between Capsicum species. Biochemical Genetics. 48: 83–95.
Jin, S. et al. 2008. Detection of somaclonal variation of cotton (Gossypium hirsutum) using cytogenetics, flow cytometry and molecular markers. Plant Cell Rpt. 27:1303–1316.
Koskinen, M. et al. 2004. The benefits of increasing the number of microsatellites used in genetic population studies: an empirical perspectiva. Hereditas 141:61-67.
Kumar, D. et al. 2001. DNA profiling of disputed chilli samples (Capsicum annum) using ISSR-PCR and FISSR-PCR marker assays. Forensic Science International.116: 63 - 68.
Leal, A. et al. 2010. Efficiency of RAPD versus SSR markers for determining genetic diversity among popcorn lines, Genet. Mol. Res.9: 9 - 18.
Liu, L. et al. 2008. DNA fingerprinting and genetic diversity analysis of late-bolting radish cultivars with RAPD, ISSR and SRAP markers. Sci. Hort. 116: 240- 247.
Michelan, V. et al. 2012. Morphological and genomic characterization of Rhynchospora tenuis complex (Cyperaceae) and its taxonomic implication. 63: 775-784.
Moreno, C. et al. 2011. Diversidad morfológica en colectas de chile guajillo (Capsicum annuum L.) del centro-norte de México. Revista Chapingo Serie Horticultura. 17: 23-30.
Nascimiento, M. et al. 2010. Variation and genetic structure of Melipona quadrifasciata Lepeletier (Hymenoptera, Apidae) populations base on ISSR pattern. Genetics and Biology. 33(2): 394-397.
Nevo, E. et al. 1986. Genetic diversity and environmental associations of wild barley, Hordeum spontaneum, in Turkey. Genetica. 68: 203-213.
Oliveira, E. et al. 2010. Optimizing the efficiency of the touchdown technique for detecting intersimple sequence repeat markers in corn (Zea mays). Genet. Mol. Res. 9: 835 - 842.
Palevitch, D. 1991. Agronomy applied to medicinal plant conservation. In: The conservation of medicinal plants. Ed. by Akerele O., Heywood V., Synge H. Cambridge University Press. Cambridge. p. 167-178.
Paran, L.; Aftergoot, E. y Shifriss, C. 1998. Variation in Capsicum annum revealed by RAPD and AFLP markers. Euphytica. 99: 167-173.
Salaya, D. 2010. Elaboración artesanal de dos abonos líquidos fermentados y su efectividad en la producción de plántula de chile habanero (Capsicum chinense Jacq.).Tesis Maestro en Ciencias. Campus Tabasco. Colegio de Postgraduados, Cárdenas, Tabasco. p.11-12.
Sanwen, H. et al. 2001. Development of pepper SSR markers from sequence data bases. 117(2): 163-167.
Shafie, M. et al. 2011. RAPD and ISSR markers for comparative analysis of genetic diversity in wormwood capillary (Artemisia capillaris) from Negeri Sembilan, Malaysia. J. Med. Plant. Res. 5(18): 4426-4437.
Srivastava, P. et al. 2004. Genetic analysis of Morus alba through RAPD and ISSR markers. Indian Journal Biotechnology. 3: 527–532.
Vargas, E. et al. 2001. Case studies on breeding systems and its consequences for germplasm conservation: 3. Electrophoretic mobility of phaseolins in wild po pulations of Lima beans (Phaseolus lunatus L.) in the Central Valley of Costa Rica. Genetic Resources and Crop Evolution. 48: 109-120.
Xu, F. y Sun, M. 2001. Comparative analysis of phylogenetic relationships of grain Amaranths and their wild relatives (Amaranthus; Amaranthaceae) using internal transcribed spacer, Amplified Fragment Length Polymorphism and Double-primer Fluorescent Intersimple Sequence Repeat Markers. Molecular Phylogenetics and Evolution. 21: 372-387.
